Molecular Identification and Phylogenetic Analysis of Functional Food Dendrobium officinale and Its Adulterants

doi:10.7506/spkx1002-6630-201102033

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (2 ): 141-145.doi: 10.7506/spkx1002-6630-201102033

• Analysis & Detection • Previous Articles Next Articles

Molecular Identification and Phylogenetic Analysis of Functional Food Dendrobium officinale and Its Adulterants

DING Ge1,2，ZHANG Dai-zhen3，ZHANG Wei-chao4，DING Xiao-yu2,*

1. Chemical and Biological Engineering College, Yancheng Institute of Technology, Yancheng 224003, China；
2. College of Life Science, Nanjing Normal University, Nanjing 210046, China；
3. Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng 224002, China；
4. Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China

Received:2010-03-05 Revised:2010-11-30 Online:2011-01-25 Published:2011-01-10
Contact: DING Xiao-yu2 E-mail:dingge2001@163.com

Abstract

Abstract: To develop a simple and reproducible molecular method to discriminate between functional food Dendrobium officinale and its adulterants, PCR products of the matK gene were sequenced to find single nucleotide polymorphism (SNP) sites. Then allele-specific PCR primers were designed according to the identified SNP sites. The phylogeny of D. officinale and its adulterants were analyzed by Mega 3.0. It was found that D. officinale and its adulterants can be discriminated by SNP sites and allele-specific PCR primers. There was apparent genetic difference between all the samples tested. It is concluded that this method can be used to discriminate between D. officinale and its adulterants rapidly and accurately.

Key words: Dendrobium officinale, matK gene, single nucleotide polymorphism, allele-specific PCR, molecular discrimination

CLC Number:

DING Ge1,2，ZHANG Dai-zhen3，ZHANG Wei-chao4，DING Xiao-yu2,*. Molecular Identification and Phylogenetic Analysis of Functional Food Dendrobium officinale and Its Adulterants[J]. FOOD SCIENCE, 2011, 32(2 ): 141-145 https://doi.org/10.7506/spkx1002-6630-201102033

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Molecular Identification and Phylogenetic Analysis of Functional Food Dendrobium officinale and Its Adulterants

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